Dextrose instead of sugar

 

The pellicle grew nicely BUT the dextrose measurement *might not be the same as the sugar* measurement. 

Given that the structure is different, the dextrose density is very fine. 

The chemical or molecular formula for sucrose is C₁₂H₂₂O₁₁. Each sugar molecule has 12 carbon atoms, 11 oxygen atoms, and 22 hydrogen atoms. 
Dextrose = Glucose is a sugar with the molecular formula C₆H₁₂O₆.
Sugar/Sucrose = Glucose is a sugar with the molecular formula C₆H₁₂O₆.

In my observations sugar works better for Scoby growth, when adding sugar to water the consistency of the water does not change while the glucose becomes more dense almost like a syrup. The scoby never fully dried even after being treated with Heat. The texture was similar to the Maple syrup experiment

***Glucose is better mixed with hot water rather than cold water. 

Fusing Scoby with Bioplastic

 Bioplastic recipe

100 ml H20

24g gelatin

12 gr glycerin

Here is the plastic against the light

I tried putting the bioplastic over the Scoby but that didn't work since the Bioplastic is aquaphobic and it started to curved and not fuse.

Instead of adding it to the top. I added it in between 2 layers of scoby to ensure that 

 

    

FINAL RESULTS 

Synscoby part II

February 25th

Arden and I set up a New Culture of Synscoby on February 25th. On March 1st it was the pellicle was showing. We set up 4 tubes. 

Since the pellicles were fairly thin on March 1st I decided to not disturb them. There was enough liquid media on each tube just took them out of the incubator to document. (photos below)

MARCH 1st

Tube #1 - March 1st

Tube #2 - March 1st Celullose didn't bind

Tube #3 - March 1st Pellicle has multiple layers

Tube #4 - March 1st

MARCH 3rd

TUBE #1: Pellicle is completely binded

TUBE #3

TUBE #4 Pellicle never binded

TUBE #2 Had the strongest binded pellicule with several layers so I removed one layers and moved it to a new tube.

TUBE #6 - March 3rd

Basics Making Synscoby media

 

TO MAKE 50ml : 22.5 ml Optiprep + 27.5 YPS

Optiprep : OptiPrep™ is a sterile endotoxin test- ed solution of 60% iodixanol in water with a density of 1.32 g/ml. Iodixanol was developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing. Iodixanol is non-ionic, non-toxic to cells and metabolically inert.

YPS = Yeast, Peptone, Sucrose

How to grow almost anything

 I'm super excited to be part of the class.

https://news.mit.edu/2023/global-lab-teaching-practicing-synthetic-biology-0118

I'm hoping to learn how to Genetically engineered my Scoby to make circuits.

Although this article is old, I would like to make my Bacterial cellulose have a similar effect. Serve as a lamp or conductive?

https://news.mit.edu/2017/engineers-create-nanobionic-plants-that-glow-1213

Food Dye, Watercolors, testing on Bacterial celullose

 

Started with a thick wet sheet of bacterial cellulose, I washed it with lukewarm water and added some watercolors.

Then added some food dye to next to the watercolor, I purchased this food dyes from Amazon, there were very inexpensive were absorbed by the bacterial cellulose very fast.

I added some glow in the dark pigment, I do not recommend the brand as I did not see any results. I have purchased glow in the dark pigment that would result in very vibrant colors. Unfortunately this one was not visible at all. Also, the box states that is highly toxic.

Lastly  I added some gold flakes just to see if they would stick to the cellulose, they did!

Wet on the left , Dry on the right.

***The only issue I'm encountering is the that the sheets are drying and becoming very stiff.

Basics. YPS media, recipe, yeast info

 YPS

In order to grow a healthy SinScoby YPS media is needed. YPS is a type of Yeast media, the acronym stands for Yeast Peptone Sucrose.

• 5g yeast extract

• 10g peptone

• 10g sucrose or sugar

• 500ml distilled water

• First step measure everything, then put all the ingredients in a glass bottle, shake it until no lumps of dry ingredients are noticeable.  Remove the bottle cap wrap it in foil, top the bottle with a piece of foil, add the autoclave tape as it is a process indicator for steam sterilizers. Put the bottle and bottle cap in the autoclave (machine that applies high-temperature steam to sterilize objects).

Distilled water

Peptone, this one is animal origin

Since there was no sucrose available in the lab we used regular sugar.

Though autoclave tape may have changed color during the sterilization process, this does not necessarily mean that an item is fully sterile. The tape will change upon exposure to the desired temperature it is indicated for. However, for proper steam sterilization to occur, the entire item needs to reach and maintain a temperature of 121 degrees Celsius for 15 to 20 minutes to ensure the items' sterility.

Note that autoclave tape and the indicators do not prove that the sterilization process has killed organisms. Instead, they demonstrate that the autoclave has reached a temperature of 121 degrees Celsius. Environmental, Health and Safety recommends that you do not use autoclave tape as the only indicator of decontamination or sterilization.

When using autoclave tape, attach it to the exterior of the item you want to sterilize. Depending on the kind of autoclave tape you use, the tape will change to a specific color.

Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced. They are relatively easy to grow under laboratory conditions. Moreover, despite their small genome size, they display cellular features and processes that are highly conserved amongst most eukaryotes. For instance, they have membrane-bound organelles, cytoskeleton, nuclear DNA, and transcription mechanisms that are similar to those found in higher eukaryotes. Furthermore, yeasts have many well-characterized secretory proteins and pheromones. Several yeast genes involved in protease processing and secretion have also been identified. Thus, yeasts can be used for several eukaryotic gene and protein studies with the aid of suitable molecular biology tools. Some applications for yeast cultures include synthesis of protein expression systems, the study of specific gene or protein functions, and the analyses of novel protein interactions.

YEAST MEDIA OPTIONS

1. YPD medium
A nutritious medium available in liquid (broth) or solid (agar) forms for the growth and propagation of yeast cultures. It primarily contains of bacteriological peptone, yeast extract, and glucose. This medium is non-selective for Candida, Pichia, Saccharomyces, and Zygosaccharomyces.

2. Yeast Synthetic Drop-out Medium Supplements
The selection of plasmids in yeast is usually based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (like an amino acid, purine, or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Thus, drop-out medium supplements can be used to select transformed yeast cultures.
Please refer to the selection guide below for further details.

3. Yeast Nitrogen Base (YNB with or without amino acids)
Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial medium contains nitrogen, vitamins, trace elements, and salts. It is suitable for use in classifying yeasts based on amino acid and carbon requirements. With an added carbon source, YNB with amino acids can be used for susceptibility testing of fungi.

Source

Yeasty boys

 Added some dry yeast. on top of the a growing scoby. I was inspired by the traditional painting with yeast. I wanted to use the powder as a way to observed how the yeast would react to the scoby environment. I added dry instant yeast,

Results, nice vibrant orange

Final result