TO MAKE 50ml : 22.5 ml Optiprep + 27.5 YPS
Optiprep : OptiPrep™ is a sterile endotoxin test- ed solution of 60% iodixanol in water with a density of 1.32 g/ml. Iodixanol was developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing. Iodixanol is non-ionic, non-toxic to cells and metabolically inert.
YPS = Yeast, Peptone, Sucrose