Growing critters ins Hestrin Schramm HS {brown media) (look recipe)
HS Recipe x 500 ml
HS
- 2.5g yeast extract
- 2.5g peptone
- 1.35g sodium phosphate
- 0.75g citric acid
- 10g sucrose
- 500ml distilled water
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Pour from the stock to a separate tube is done in the sterile hood
Streaking from Plates. with the microbes from (Part1) :
Taking a loopful of microbe culture (a colony) from the plates and putting them in Media
October 25 LEFT November 2nd RIGHT pellicle is visible - grew in 30C shaker
November 2nd
We run a test to see which tube top worked better a snap off cap and a screw top
The Snap off seems to be better as it lets more air come in
November 4th - moving forward we will only use Snap off tops
Nov7 - Testing to see if the K. Rhaeticus we took from the frozen stock will grow on HS media
We just took a bit from the frozen stock that we put in the -80C
Growing the pellicle in motion or still. Both will be growing at 30C, One in the shaker and one in the incubator
Nov 14 , The one on the shaker grew, the one in the incubator did not grew.
