Synscoby part II

February 25th

Arden and I set up a New Culture of Synscoby on February 25th. On March 1st it was the pellicle was showing. We set up 4 tubes. 

Since the pellicles were fairly thin on March 1st I decided to not disturb them. There was enough liquid media on each tube just took them out of the incubator to document. (photos below)

MARCH 1st

Tube #1 - March 1st

Tube #2 - March 1st Celullose didn't bind

Tube #3 - March 1st Pellicle has multiple layers

Tube #4 - March 1st

MARCH 3rd

TUBE #1: Pellicle is completely binded

TUBE #3

TUBE #4 Pellicle never binded

TUBE #2 Had the strongest binded pellicule with several layers so I removed one layers and moved it to a new tube.

TUBE #6 - March 3rd

Basics Making Synscoby media

 

TO MAKE 50ml : 22.5 ml Optiprep + 27.5 YPS

Optiprep : OptiPrep™ is a sterile endotoxin test- ed solution of 60% iodixanol in water with a density of 1.32 g/ml. Iodixanol was developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing. Iodixanol is non-ionic, non-toxic to cells and metabolically inert.

YPS = Yeast, Peptone, Sucrose